MED12 Regulates HSC-Specific Enhancers Independently of Mediator Kinase Activity to Control Hematopoiesis
نویسندگان
چکیده
منابع مشابه
The human CDK8 subcomplex is a histone kinase that requires Med12 for activity and can function independently of mediator.
The four proteins CDK8, cyclin C, Med12, and Med13 can associate with Mediator and are presumed to form a stable "CDK8 subcomplex" in cells. We describe here the isolation and enzymatic activity of the 600-kDa CDK8 subcomplex purified directly from human cells and also via recombinant expression in insect cells. Biochemical analysis of the recombinant CDK8 subcomplex identifies predicted (TFIIH...
متن کاملThe MED12-MED13 module of Mediator regulates the timing of embryo patterning in Arabidopsis.
The Arabidopsis embryo becomes patterned into central and peripheral domains during the first few days after fertilization. A screen for mutants that affect this process identified two genes, GRAND CENTRAL (GCT)and CENTER CITY (CCT). Mutations in GCT and CCT delay the specification of central and peripheral identity and the globular-to-heart transition, but have little or no effect on the initi...
متن کاملMED12 in hematopoietic stem cells-cell specific function despite ubiquitous expression.
All cells contain several multi-component molecular machines for executing steps in the fundamental cellular processes of transcription and translation. Paradoxically, despite their ubiquitous expression, individual components of these protein conglomerates are responsible for extraordinarily cell-specific functions. One of these fundamental molecular complexes is Mediator. Mediator is expresse...
متن کاملThe Nucleosome Remodelling and Deacetylation complex restricts Mediator access to enhancers to control transcription
1. Wellcome Trust – Medical Research Council Stem Cell Institute, University of Cambridge, Gleeson Building, Tennis Court Road, Cambridge CB2 1QR United Kingdom 2. European Bioinformatics Institute, European Molecular Biology Laboratory (EMBL), Wellcome Trust Genome Campus, Cambridge CB10 1SD, United Kingdom 3. Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB...
متن کاملMED12 regulates a transcriptional network of calcium-handling genes in the heart.
The Mediator complex regulates gene transcription by linking basal transcriptional machinery with DNA-bound transcription factors. The activity of the Mediator complex is mainly controlled by a kinase submodule that is composed of 4 proteins, including MED12. Although ubiquitously expressed, Mediator subunits can differentially regulate gene expression in a tissue-specific manner. Here, we repo...
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ژورنال
عنوان ژورنال: Cell Stem Cell
سال: 2016
ISSN: 1934-5909
DOI: 10.1016/j.stem.2016.08.004